Supplemental Quiz

Review the methodology defining the polymerase chain reaction. Answer the following 3 questions.

1. The most likely reason for lowering the temperature to 55^oC is?

A. To minimize hydrogen bonding of primer to template.
B. To maximize hydrogen bonding of primer to template.
C. To minimize nucleoside triphosphate binding to the template.
D. To maximize nucleoside triphosphate binding to the template.
E. To reanneal (re-join) the double-stranded DNA.

2. The DNA is heated to effectively substitute for the cellular ____________.

A. helicase
B. DNase
C. ligase
D. primase
E. topoisomerase

3. The origin of replication in a PCR reaction is?

A. Not needed
B. Defined by the primer
C. On the native DNA template
D. Exposed at 95^oC
E. Denatured at 95^oC

4. Describe how DNA fingerprinting was used to determine Thomas Jefferson's promiscuity.

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