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1 | |
The use or alteration of cells or biochemicals to provide a useful product describes |
| | A) | recombinant DNA technology. |
| | B) | transgenic technology. |
| | C) | biotechnology. |
| | D) | gene targeting. |
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2 | |
Which of the technologies listed below is a valuable method for mass-producing drugs and other useful proteins? |
| | A) | recombinant DNA technology |
| | B) | transgenic technology |
| | C) | biotechnology |
| | D) | gene targeting |
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3 | |
Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location? |
| | A) | restriction enzyme |
| | B) | plasmid |
| | C) | bacteriophage |
| | D) | vector |
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4 | |
Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the below describe the circular piece of DNA from a bacterial cell? |
| | A) | restriction enzyme |
| | B) | plasmid |
| | C) | bacteriophage |
| | D) | vector |
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5 | |
Which gene transfer technique involves a tiny needle which is used to inject DNA into a cell lacking that DNA sequence? |
| | A) | electroporation |
| | B) | liposome transfer |
| | C) | microinjection |
| | D) | particle bombardment |
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6 | |
The process of ____ involves the introduction of a gene into a cell where it exchanges places with its counterpart in the host cell. |
| | A) | transgenic technology |
| | B) | gene targeting |
| | C) | knockout technology |
| | D) | recombinant DNA technology |
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7 | |
A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is a |
| | A) | transversion. |
| | B) | transition. |
| | C) | transgenic. |
| | D) | transformant. |
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8 | |
The first drug produced using recombinant DNA technology is used to treat |
| | A) | hemophilia. |
| | B) | dwarfism. |
| | C) | heart attack. |
| | D) | diabetes. |
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9 | |
Genetic engineering manipulates gene products at the level of the |
| | A) | protein. |
| | B) | amino acid. |
| | C) | DNA. |
| | D) | RNA. |
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10 | |
A knockout mouse is so named because |
| | A) | it contains aggression genes. |
| | B) | it contains multiple copies of a human gene. |
| | C) | it contains an activated human gene. |
| | D) | none of the above. |
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11 | |
The first patent for a transgenic organism was awarded in 1988. Which organism was patented? |
| | A) | a yeast used in industrial processes |
| | B) | a bacterium able to metabolize components of crude oil |
| | C) | a mouse that manufactures human protein in its milk |
| | D) | life forms cannot be patented |
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12 | |
Palindromic sequences in DNA |
| | A) | form "blunt" ends when cut by restriction enzymes. |
| | B) | reflect the same sequence on two sides. |
| | C) | are not useful in recombinant DNA experiments. |
| | D) | all of the above. |
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13 | |
A cDNA version of a gene includes |
| | A) | codons for a mature mRNA. |
| | B) | sequences corresponding to promoters. |
| | C) | sequences corresponding to introns. |
| | D) | both b and c. |
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14 | |
Which of the following transgenic pharming products is incorrectly paired with the host organism that produces it? |
| | A) | hemoglobin - rabbit |
| | B) | human growth hormone - rat |
| | C) | lactoferrin - cows |
| | D) | alpha-1-antitrypsin - sheep |
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15 | |
Swapping an inactivated allele for a gene of interest produces a ____ mouse while replacing a gene with another that has an altered function creates a ____ mouse. |
| | A) | knockout, knockout |
| | B) | knockout, gene targeted |
| | C) | knockout, knockin |
| | D) | gene targeted, knockin |
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16 | |
Gene targeting is done on a(n) |
| | A) | sperm cell. |
| | B) | egg cell. |
| | C) | fertilized ovum. |
| | D) | early embryonic cell. |
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17 | |
Human proteins can be produced in the milk or semen of farm animals. True or false? |
| | A) | true |
| | B) | false, proteins cannot be produced in milk |
| | C) | false, proteins cannot be produced in semen |
| | D) | false, animals are not used for protein production |
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18 | |
____ consist of recombinant cells containing different fragments of a foreign genome. |
| | A) | DNA probes |
| | B) | Homologous recombinants |
| | C) | Genomic libraries |
| | D) | Knockout organisms |
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19 | |
Recombinant DNA technology methods began in the |
| | A) | 1950's. |
| | B) | 1960's. |
| | C) | 1970's. |
| | D) | 1980's. |
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20 | |
____ are used to select genes of interest from a genomic library. |
| | A) | Restriction enzymes |
| | B) | Cloning vectors |
| | C) | DNA probes |
| | D) | Gene targets |
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21 | |
For the same gene, it is possible to patent |
| | A) | expressed sequence tags |
| | B) | cDNA |
| | C) | SNPs |
| | D) | all of the above |
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22 | |
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell? |
| | A) | electroporation |
| | B) | liposome transfer |
| | C) | microinjection |
| | D) | particle bombardment |
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23 | |
"Naked" DNA |
| | A) | is free of nucleic acids. |
| | B) | is free of the cell. |
| | C) | is free of protein. |
| | D) | contains just the sugar-phosphate backbone. |
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24 | |
Which of the following gene-targeted mice have shown that a gene product once thought important is not vital to survival? |
| | A) | neurofibromatosis |
| | B) | X collagen |
| | C) | SCID |
| | D) | sickle-cell |
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25 | |
The first drug produced using recombinant DNA technology was |
| | A) | streptokinase. |
| | B) | tPA. |
| | C) | insulin. |
| | D) | penicillin. |
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