First Experiment

1		What is the purpose of adding DNase to the cell extract?
			A)	To digest the bacterial DNA thereby preventing the formation of new mRNA
			B)	To digest the bacterial DNA thereby preventing DNA replication
			C)	To digest the bacterial mRNA
			D)	To enhance the formation of mRNA
2		If the addition of trichloroacetic acid was inadvertently omitted, how would that affect the results?
			A)	The results would not be affected.
			B)	There would be more radioactivity on the filter.
			C)	There would be less radioactivity on the filter.
			D)	None of the above
3		If a random polymer was made from nucleotides containing 40% A and 60% G, what codons would be made and in what proportions?
			A)	6.4% AAA, 9.6% AAG, 9.6% AGA, 14.4% AGG, 9.6% GAA, 14.4% GGA, 14.4% GAG, and 21.6% GGG
			B)	40% AAA and 60% GGG
			C)	16% AAG, 9.6% AGA, 14.4% AGG, 9.6% GAA, 14.4% GGA, and 36% GAG
			D)	None of the above
4		If a random RNA polymer was made from 40% A and 60% G, what amino acids would be found in the resulting polypeptide and in what proportions?
			A)	Lysine (16%), alanine (24%), arginine (24%), and glycine (36%)
			B)	Leucine (16%), glutamic acid (24%), arginine (24%), and glycine (36%)
			C)	Lysine (16%), alanine (24%), glutamic acid (24%), and glycine (36%)
			D)	Lysine (16%), glutamic acid (24%), arginine (24%), and glycine (36%)
5		If a polymer was made from nucleotides containing A, G, and C, what amino acids would be found in the resulting polypeptide?
			A)	Glutamine, arginine, threonine, serine, alanine, and aspartic acid
			B)	Proline, histidine, glutamine, arginine, threonine, asparagine, lysine, serine, alanine, aspartic acid, glutamic acid, and glycine
			C)	Leucine, proline, histidine, glutamine, and arginine
			D)	Valine, alanine, aspartic acid, glutamic acid, and glycine

Second Experiment

6		In this experiment, the bacterial cell extract contained
			A)	All types of tRNAs
			B)	Ribosomes
			C)	Aminoacyl tRNA-synthetases
			D)	All of the above
7		In this experiment, 14C was used to radiolabel cysteine. If the same amount of 35S had been used to label cysteine instead of 14C, how would the results have been different?
			A)	The results would have been the same.
			B)	There would have been less radioactivity in the Raney nickel-treated, alanine fraction.
			C)	There would have been more radioactivity in the control, cysteine fraction.
			D)	There would have been more radioactivity in the control, alanine fraction.
8		A polyUG mRNA contains
			A)	Cysteine codons
			B)	Alanine codons
			C)	Methionine codons
			D)	Tyrosine codons
9		The purpose of the 15% trichloroacetic acid (TCA) step is to
			A)	Promote the formation of polypeptides
			B)	Precipitate polypeptides
			C)	Precipitate free amino acids
			D)	Denature the RNA polymer
10		If TCA had been accidentally omitted from the experiment, how would the results have been affected?
			A)	There would have been no effect.
			B)	There would have been more radioactivity on the filter.
			C)	There would have been less radioactivity on the filter.
			D)	There would have been less radioactivity passing through the filter.