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Study Outline
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  1. Transcriptional Regulation
    1. The phenomenon of enzyme adaptation is due to the synthesis of cellular proteins
    2. The lac operon encodes proteins that are involved in lactose metabolism
    3. The lac operon is regulated by a repressor protein
    4. Bacterial conjugation experiments provide evidence that the lacI gene encodes a repressor protein
    5. The lac operon is also regulated by an activator protein
    6. Further studies have revealed that the lac operon has three operator sites for the lac repressor
    7. The ara operon can be regulated positively or negatively by the same regulatory protein
    8. The trp operon is regulated by a repressor protein and also by attenuation
    9. Repressible operons usually encode anabolic enzymes, and inducible operons encode catabolic enzymes
  2. Translational and Posttranslational Regulation
    1. Repressor proteins and antisense RNA can inhibit translation
    2. Posttranslational regulation can occur via feedback inhibition and covalent modifications
  3. Gene Regulation In The Bacteriophage Life Cycle
    1. Phage l can follow a lytic or lysogenic life cycle
    2. During the lysogenic cycle, the cII/cIII complex activates expression of the l repressor
    3. The lytic cycle depends on the action of the cro protein
    4. Cellular proteases influence the choice between the lytic and lysogenic cycle
    5. The OR region provides a genetic switch between the lytic and lysogenic cycles





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