First Experiment

1		The reason why a mutant gene is likely to be associated with a particular RFLP is because
			A)	The mutation in the gene changes a restriction site.
			B)	The mutation in the gene is a deletion.
			C)	The original mutation occurred in a single founder, and everyone has RFLPs that are within or closely linked to particular genes.
			D)	None of the above
2		The purpose of adding HpaI was to
			A)	Denature the DNA
			B)	Digest the DNA into short fragments
			C)	Link the β-globin gene to an RFLP
			D)	Separate the β-globin gene from an RFLP
3		In this experiment, the Southern blotting procedure detected
			A)	A DNA fragment of the β-globin gene
			B)	The mutation within the β-globin gene
			C)	Only the RFLP, but not the β-globin gene
			D)	None of the above
4		If an individual was 13.0/7.0, it would be most likely for that individual to be
			A)	Homozygous for the sickle cell allele
			B)	Homozygous for the normal allele
			C)	Heterozygous for the two alleles
			D)	None of the above
5		It is occasionally possible for an individual to carry the sickle-cell allele and yet not have the 13.0 RFLP. If the founder hypothesis is correct, what is the most likely explanation?
			A)	A second mutation created a new HpaI site.
			B)	A second mutation eliminated an HpaI site.
			C)	A second mutation caused a deletion.
			D)	In a heterozygous individual, a crossover occurred between the original mutation (in a 13.0 individual) and an HpaI site in a 7.0 or 7.6 individual.