PCR Reactions

View the animation below, then complete the quiz to test your knowledge of the concept.

1		PCR requires all of the following EXCEPT
			A)	primers.
			B)	DNA ligase.
			C)	DNA polymerase.
			D)	DNA of interest.
			E)	deoxyribonucleotides.
2		During PCR, which primer anneals to DNA with its 3’ end towards the center of the sequence to be amplified?
			A)	forward primer only
			B)	reverse primer only
			C)	both the forward and reverse primers
			D)	either the forward or reverse primer, but not both
			E)	neither the forward nor reverse primer
3		Arrange the following in the proper sequence in which they occur during a single PCR cycle. 1. Addition of DNA nucleotides by Taq polymerase 2. Complementary base pairing between primers and target DNA 3. Heat separation of strands of target DNA
			A)	1, 2, 3
			B)	2, 1, 3
			C)	2, 3, 1
			D)	3, 2, 1
			E)	3, 1, 2
4		All double-stranded DNA molecules generated by PCR are identical.
			A)	True
			B)	False
5		PCR is carried out in machines termed thermocyclers.
			A)	True
			B)	False