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1
Genetic engineering has successfully transferred genes from eukaryotic cells into
A)bacteria.
B)plants.
C)animals.
D)All of the above
2
DNA molecules can be cut into sections by using
A)ATP.
B)gel electrophoresis.
C)restriction enzymes.
D)plasmids.
3
In genetic engineering, ligase is used as
A)a probe.
B)a sealing enzyme.
C)a restriction enzyme.
D)a mutagen.
4
A probe is used in which stage of the gene transfer process?
A)Cleaving DNA
B)Recombining DNA
C)Cloning
D)Screening
5
Polymerase chain reactions are used to
A)create DNA "fingerprints."
B)cleave bacterial plasmids.
C)seal "sticky ends."
D)identify target plasmids.
6
Genetic engineering has been used to do all of the following except
A)make plants more resistant to frost.
B)make plants more resistant to disease.
C)make plants more resistant to herbicides.
D)All of the above are correct.
7
Engineering plants that are resistant to glyphosate was an important advancement because
A)glyphosate promotes frost damage.
B)glyphosate encourages the production of fruit that is lower in protein.
C)glyphosate is the active ingredient in herbicide.
D)glyphosate prevents the transfer of genes into the plants.
8
The use of bovine growth hormone has
A)raised controversy with groups opposed to genetic engineering.
B)increased milk production.
C)increased the weight gain of cows.
D)resulted in both 1 and 2.
9
The polymerase chain reaction, or PCR, is a procedure used to chop DNA into smaller subunits.
A)True
B)False
10
Interferons are used to disrupt the reproduction of viruses.
A)True
B)False
11
Radioactively labeled probes are used to screen bacterial colonies for the presence of a desired gene.
A)True
B)False
12
The second stage in gene transfer is cloning the plasmid.
A)True
B)False
13
Factor VIII is a protein found in anticoagulants used to treat heart attack patients.
A)True
B)False
14
Genetic engineering can make plants toxic to insect pests that feed on the plant, but leave the plant harmless to humans.
A)True
B)False
15
Genetic engineering has produced salmon
A)with shorter reproductive cycles.
B)that produce four times more offspring.
C)resistant to viral infection.
D)that are heavier than non-engineered salmon.
E)both 1 and 4
16
Sticky ends are the result of
A)treatment of a nucleotide sequence with DNA ligase.
B)exposure of eukaryotic DNA to a prokaryotic plasmid.
C)infection of a cell with a bacteriophage.
D)DNA breaking down in the presence of reverse transcriptase.
E)cutting by restriction enzymes "off center" in a specific nucleotide sequence.
17
In order to produce a DNA fragment that can be inserted into the DNA of a second organism, one would need to
A)create a compatible DNA segment from an mRNA template.
B)find a virus that could serve as a vector for transfer of the donor DNA.
C)make sure both organisms have compatible DNA.
D)be sure both donor and recipient DNA were prokaryotic or eukaryotic.
E)cut the DNA of the donor and recipient cells with the same restriction enzyme.
18
One possible risk of genetic engineering is the accidental production of dangerously resistant microorganisms.
A)True
B)False
19
Vaccines are developed through the use of genetic engineering.
A)True
B)False
20
The development of "golden" rice will help prevent which nutritional deficiency?
A)Vitamin A
B)Calcium
C)Iron
D)Vitamin C
E)Vitamin D





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