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1The manipulation of organisms for practical purposes is ________.
A)molecular biology
B)genetic engineering
C)biotechnology
D)genetic transformation



2The direct manipulation of genes for practical purposes is ________.
A)molecular biology
B)genetic engineering
C)biotechnology
D)genetic transformation



3The study of molecules critical to life is ________.
A)molecular biology
B)genetic engineering
C)biotechnology
D)genetic transformation



4What two techniques will you learn in today's lab?



5From what organism will you be isolating DNA?
A)wheat germ
B)banana
C)bacterium
D)cheek cell



6DNA denatures in high heat or alkaline pH.
A)True
B)False



7The uptake and expression of DNA by a living cell is ________.
A)molecular biology
B)genetic engineering
C)biotechnology
D)genetic transformation



8Which condition is required for gene transformation?
A)a way of inserting DNA into a host
B)a method for identifying transformed organisms
C)a host into which DNA can be inserted
D)all of the above



9What host organism will you use in the genetic transformation experiment?



10E. coli is well-suited for genetic transformation studies because it has only one chromosome and it multiplies rapidly.
A)True
B)False



11A DNA molecule that carries DNA sequences into a host is a biological ___________.
A)vehicle
B)vector
C)courier
D)parasite



12Circular pieces of DNA made of 1000 to 200,000 base pairs that are separate from a bacterium's main circle of DNA are called ________.
A)plasmids
B)plasmas
C)chromatids
D)circulon



13In your study of genetic transformation, bacteria growing on your nutrient medium containing ampicillin were transformed.
A)True
B)False



14Heat shock decreases the uptake of the plasmid by the host cells.
A)True
B)False



15Once isolated, DNA can be sequenced, manipulated and altered.
A)True
B)False







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