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1What are the three types of techniques that you will use in this lab to separate organic compounds?


2The apparatus used for column chromatography consists of what three parts?


3What determines the range of molecular weights that a matrix can separate?


4In column chromatography, what is the function of the buffer?


5Larger molecules move faster in column chromatography.
A)True
B)False


6In paper chromatography, the _______ phase is an organic solvent that moves along the paper, and the ________ phase is the paper filter.


7In paper chromatography, what characteristics of organic molecules affect their rate of movement?


8What is the formula for the retardation factor, Rf?


9What does the word chromatography mean?


10Gel electrophoresis separates molecules according to their _______.
A)electrical charge
B)shape
C)size
D)all of the above


11Larger fragments move faster in gel electrophoresis.
A)True
B)False


12The apparatus for gel electrophoresis consists of what five parts?


13What is the function of the power supply in gel electrophoresis?


14DNA has a negative charge. Toward which electrode will DNA move?


15Small, spherical molecules move slower than large, rod-like molecules in a gel.
A)True
B)False


16Particles with the greatest charge move faster than particles with lesser charge in a gel.
A)True
B)False





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