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Animation Quiz 2 - Polymerase Chain Reaction
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Review the animation and answer the questions that follow to test your understanding.



1

The purpose of PCR is to
A)make more copies of DNA primers to increase protein synthesis
B)make many copies of an organism’s DNA sequence so a small number of organisms will become large enough to be identified
C)make more RNA so large units of protein can be synthesized
D)recycle DNA using thermocyclers
2

For DNA amplification to occur, which of the following are needed?
A)loose ribonucleotides
B)RNA primers
C)thermostable DNA polymerase
D)b and c
E)all of the above
3

Taq polymerase starts copying at
A)the end of free single-stranded RNA
B)any open point
C)RNA primers attached to the end of the desired gene
D)DNA primers attached to the end of the desired gene
4

Using PCR, over 1 million copies of DNA can be made from a single strand of DNA overnight.
A)True
B)False
5

When DNA is heated, primers anneal to DNA strands.
A)True
B)False







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