Review the animation and answer the questions that follow to test your understanding.
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| 1.
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In insertional inactivation |
| | A) | if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies remain white |
| | B) | if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies turn blue |
| | C) | if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies remain white |
| | D) | if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies turn blue |
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| 2.
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If host cells are ampicillin sensitive and are plated on a medium containing ampicillin |
| | A) | only cells that have not taken up the ampicillin resistant vector can grow |
| | B) | only cells that have taken up the ampicillin resistant vector can grow |
| | C) | all cells will grow |
| | D) | ampicillin is inactivated |
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| 3.
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The lac z gene marker codes for |
| | A) | b galactosidase, which splits x-gal |
| | B) | b galactosidase, which makes x-gal resistant to splitting |
| | C) | ampicillin resistance |
| | D) | white colonies |
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| 4.
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When foreign DNA and plasmid are both cut with the same restriction enzyme and mixed together, all molecules will form recombinants. |
| | A) | True |
| | B) | False |
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| 5.
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When beta galactosidase cleaves x-gal, white colonies result. |
| | A) | True |
| | B) | False |
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