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Multiple Choice Quiz
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1
A homothallic yeast strain sporulates as a haploid.
A)True
B)False
2
When a MATa/MATalpha diploid undergoes meiosis, the mating type patterns of the spores in any given tetrad should be 2 a and 2 alpha.
A)True
B)False
3
a cells mate with a cells; alpha cells mate with alpha cells; a/alpha cells do not mate.
A)True
B)False
4
Loss-of-function mutations of the MATalpha1 gene cause a defect in transcriptional activation of alpha-specific genes.
A)True
B)False
5
The Mcm1 protein is not necessary for repression of transcription of haploid-specific genes in a/alpha cells.
A)True
B)False
6
Diploids homozygous at the mating type locus are sterile.
A)True
B)False
7
A permanently inactive locus that resembles wild-type genes is:
A)homothallic
B)heterothallic
C)pheromone
D)pseudogene
E)shuttle vector
8
A strain with a stable haploid mating type is known as:
A)homothallic
B)heterothallic
C)pheromone
D)pseudogene
E)shuttle vector
9
A chemical signal that can move between cell types is:
A)homothallic
B)heterothallic
C)pheromone
D)pseudogene
E)shuttle vector
10
A DNA molecule that can be moved between cell types is:
A)homothallic
B)heterothallic
C)pheromone
D)pseudogene
E)shuttle vector
11
A strain with a switchable mating type is:
A)homothallic
B)heterothallic
C)pheromone
D)pseudogene
E)shuttle vector
12
What would be the most suitable subject for study in yeast?
A)photosynthesis
B)mouse mating behavior
C)cell cycle regulation
D)chemotaxis
E)organogenesis (organ development)
13
Which of the following statements about yeast chromosomes is not true?
A)they vary in size from less than 300 kilobases to as much as 3000 kilobases
B)they can be physically separated by pulsed-field gel electrophoresis techniques
C)each contains a centromere, two telomeres, and multiple origins of replication
D)they contain the 2 micron origin of replication
E)have a longer linkage map than N. crassa
14
Which of the following statements about the yeast genome is not true?
A)haploid chromosome number is 16
B)relative distances of genes on the genetic and physical maps may differ
C)relative order of genes on the genetic and physical maps may differ
D)the genetic map was determined by counting meiotic crossovers
E)the physical map was determined by gene sequencing
15
Properties of Ty elements include all but:
A)the ability to transpose within a yeast cell
B)superficial similarity to animal retroviruses
C)the ability to recombine with other elements at nonhomologous positions
D)a lytic cycle
E)5.4-6.3 kb in length
16
Sterile mutants that fail to respond to pheromone are likely to have a defect in:
A)one of the structural genes encoding the pheromone peptide
B)a gene encoding a protease required for cleavage of the pre-pro-pheromone
C)a gene encoding a protease required for cleavage of a leader sequence
D)an STE gene
E)URA3
17
A yeast strain has a defect in G proteins involved in the mating system. What property(ies) does this mutant have?
A)produces pheromones
B)arrests in diploid stage
C)can switch mating type
D)a & b
E)a & c
18
What is true of a and alpha pheromone?
A)modified post-translationally
B)synthesized in response to opposite pheromone
C)is secreted
D)a & c
E)all of the above
19
Silencing of the storage loci HML and HMR:
A)promotes transcription from genes at these loci
B)is mediated through alterations in heterochromatin
C)allows cleavage by the HO endonuclease at these loci
D)all of the above
E)none of the above
20
Which of the following statements about the pattern of HO-mediated mating type switching is not true?
A)involves double strand breakage and repair
B)site of action is on chromosome III
C)following sporulation, half of the spores switch mating type in an HO/HO diploid
D)cells cannot switch until they have divided once
E)Mating type does not switch in diploid
21
A haploid strain with a mutation in one of the SIR genes can mate with either a or alpha strains.
A)True
B)False
22
In mating type switching, the HML locus acts as a recipient.
A)True
B)False
23
Nonviable spores cannot be counted in tetrad analysis.
A)True
B)False
24
Loss-of-function mutations of the MATa1 gene cause no phenotype in haploids.
A)True
B)False
25
Loss-of-function mutations of the MATalpha2 gene cause simultaneous expression of both a- and alpha-functions.
A)True
B)False
26
A site-specific endonuclease is encoded by:
A)MAT
B)HML
C)MFa1
D)HO
E)STE3
27
An inactive copy of mating information is carried at:
A)MAT
B)HML
C)MFa1
D)HO
E)STE3
28
The active, or type-determining mating information is carried at:
A)MAT
B)HML
C)MFa1
D)HO
E)STE3
29
A mating pheromone signal transduction protein is encoded by:
A)MAT
B)HML
C)MFa1
D)HO
E)STE3
30
A mating pheromone is encoded by:
A)MAT
B)HML
C)MFa1
D)HO
E)STE3
31
You starve a yeast culture for an extended time. Which of the following would you find in the culture?
A)haploid vegetative cells
B)ascospores
C)pheromones
D)a & c
E)all of the above
32
Mating type switching occurs in cells of genotype:
A)HO/HO MATa/MATa.
B)HO MATalpha
C)HO/HO MATa/MATalpha
D)HO/HO MATalpha/MATalpha
E)none of the above
33
Which of the following plasmids cannot replicate in yeast cells?
A)YIp
B)YEp
C)YCp
D)a & b
E)a & c
34
Which of the following plasmids has a copy number of one?
A)YIp
B)YEp
C)YCp
D)a & b
E)a & c
35
You are attempting to make a URA+ strain by gene replacement. Which step is in error?
A)Obtain a plasmid bearing a cloned copy of the gene of interest
B)Use restriction enzymes to cut out part of the coding region of the gene of interest
C)Ligate a DNA fragment containing the URA-52 mutation gene to the 5' and 3' regions of the gene of interest; transform into E. coli and select for antibiotic resistance; screen for ligation products with the appropriate restriction map
D)Transform yeast with a linear DNA fragment containing the replacement allele and select on medium lacking uracil
E)Screen the uracil-independent colonies for phenotype
36
Which of the following functions in one-step gene disruption/replacement?
A)homologous recombination
B)illegitimate recombination
C)plasmid integration
D)a & c
E)b & c
37
Which of the following genes are expressed in a/alpha diploids?
A)a-specific genes
B)STE4
C)STE18
D)all of the above
E)none of the above
38
By using homologous recombination of flanking sequences, which of the following genetic constructions may be made in yeast?
A)deletions
B)introduction of heterologous sequences
C)gene replacement
D)all of the above
E)none of the above
39
Pulsed field gel electrophoresis of chromosomes in yeast can detect:
A)inversions
B)translocations within a chromosome
C)translocation between chromosomes
D)substitutions
E)banding patterns
40
Of the following, which is not involved in the mating system in yeast?
A)G proteins
B)illegitimate recombination
C)homologous recombination
D)transcription factors
E)cell surface receptors





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